nebnext multiplex oligos for illum (New England Biolabs)
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New England Biolabs
nebnext multiplex oligos for illum
Nebnext Multiplex Oligos For Illum, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+multiplex+oligos/NEBNext+Multiplex+Oligos+for+Illumina/pmc13651819-162-0-0
Average 96 stars, based on 2193 article reviews
Nebnext Multiplex Oligos For Illum, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+multiplex+oligos/NEBNext+Multiplex+Oligos+for+Illumina/pmc13651819-162-0-0
Average 96 stars, based on 2193 article reviews
nebnext multiplex oligos for illum - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses"
Article Title: Gapmers targeting conserved SARS-CoV-2 single-stranded regions reduce viral load and reshape inflammatory macrophage responses
Journal: iScience
doi: 10.1016/j.isci.2026.117639
Figure Legend Snippet:
Techniques Used: Virus, Variant Assay, Recombinant, Reverse Transcription, Diagnostic Assay, Luminex, Ligation, Multiplex Assay, Generated, Software
Related Articles
Purification:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Polymerase Chain Reaction:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA. Article Snippet: .. Preparation of Illumina libraries and NGS sequencing Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA Article Snippet: .. Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Multiplex Assay:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Article Title: Molding the Rice Methylome for Disease Resistance Article Snippet: High-purity genomic DNA was extracted using IllustraTM NucleonTM PhytoPureTM kit (Cytiva), then sheared to ∼500bp fragments through sonication using Covaris M220. .. Enzymatic conversion of methylated cytosines and library preparation were performed using NEBNext® Enzymatic Methyl-seq Kit and Article Title: Alu-mediated RNA duplexes are associated with widespread exon skipping across primate transcriptomes Article Snippet: .. Reverse transcription was performed with SuperScript IV at 50 °C for 1 h using 1 μM 3′ adaptor–specific primer (5’ -TTCAGACGTGTGCTCTTCCGATCTATTGATGGTGCCTACAG-3’) and 1 μM template-switch oligo (TSO) containing a 10-nt unique molecular identifier (UMI) (5’ -CTT TCC CTA CAC GAC GCT CTT CCG ATCT NNNNNNNNNN TAC rGrGrG/3phos/−3’). cDNA fragments of 150–300nt were size-selected on a 6% urea-PAGE gel, then amplified with Article Title: Epigenetic control of S100A8/A9-driven monocytic inflammation licenses anti-leukemic functionality of immature NK cells during hematopoietic stem cell differentiation Article Snippet: .. Libraries were generated from total RNA after poly(A)^+^ mRNA enrichment using the NEBNext Ultra II Directional RNA Library Prep Kit with Article Title: Cancer detection and classification using methylome analysis Article Snippet: Briefly, for MeDIP, the Diagenode MagMeDIP kit (Cat #C02010021) was used following the manufacturer's protocol with some modifications. .. The optimal number of cycles to amplify each library was determined through the use of qPCR, after which the samples were amplified using the KAPA HiFi Hotstart Mastermix and the Article Title: deluxpore: a Nextflow pipeline for demultiplexing Illumina dual-indexed Nanopore libraries Article Snippet: .. As a solution, target enrichment can be performed on Illumina-indexed libraries, whereby dual indexes incorporated during library preparation allow samples to be pooled prior to capture hybridisation, after which the enriched pool is converted into a Nanopore-compatible library for MinION sequencing; a strategy recently applied to microbial gene capture using Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA. Article Snippet: .. Preparation of Illumina libraries and NGS sequencing Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA Article Snippet: .. Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Sequencing:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Article Title: deluxpore: a Nextflow pipeline for demultiplexing Illumina dual-indexed Nanopore libraries Article Snippet: .. As a solution, target enrichment can be performed on Illumina-indexed libraries, whereby dual indexes incorporated during library preparation allow samples to be pooled prior to capture hybridisation, after which the enriched pool is converted into a Nanopore-compatible library for MinION sequencing; a strategy recently applied to microbial gene capture using Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA. Article Snippet: .. Preparation of Illumina libraries and NGS sequencing Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Incubation:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Reverse Transcription:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Article Title: Alu-mediated RNA duplexes are associated with widespread exon skipping across primate transcriptomes Article Snippet: .. Reverse transcription was performed with SuperScript IV at 50 °C for 1 h using 1 μM 3′ adaptor–specific primer (5’ -TTCAGACGTGTGCTCTTCCGATCTATTGATGGTGCCTACAG-3’) and 1 μM template-switch oligo (TSO) containing a 10-nt unique molecular identifier (UMI) (5’ -CTT TCC CTA CAC GAC GCT CTT CCG ATCT NNNNNNNNNN TAC rGrGrG/3phos/−3’). cDNA fragments of 150–300nt were size-selected on a 6% urea-PAGE gel, then amplified with Nested PCR:Article Title: DNA-PKcs inhibitor AZD7648 reveals sgRNA cross-contaminants and enhanced sensitivity of genome engineering off-target activity in HSPCs. Article Snippet: .. The purified PCR amplicons were hen end repaired, A-tailed, ligated to an adapter, and purified ollowing the protocol in NEBNext ® UltraTM II DNA Library rep Kit for Illumina ® and Methylation:Article Title: Molding the Rice Methylome for Disease Resistance Article Snippet: High-purity genomic DNA was extracted using IllustraTM NucleonTM PhytoPureTM kit (Cytiva), then sheared to ∼500bp fragments through sonication using Covaris M220. .. Enzymatic conversion of methylated cytosines and library preparation were performed using NEBNext® Enzymatic Methyl-seq Kit and Size Selection:Article Title: Molding the Rice Methylome for Disease Resistance Article Snippet: High-purity genomic DNA was extracted using IllustraTM NucleonTM PhytoPureTM kit (Cytiva), then sheared to ∼500bp fragments through sonication using Covaris M220. .. Enzymatic conversion of methylated cytosines and library preparation were performed using NEBNext® Enzymatic Methyl-seq Kit and Amplification:Article Title: Alu-mediated RNA duplexes are associated with widespread exon skipping across primate transcriptomes Article Snippet: .. Reverse transcription was performed with SuperScript IV at 50 °C for 1 h using 1 μM 3′ adaptor–specific primer (5’ -TTCAGACGTGTGCTCTTCCGATCTATTGATGGTGCCTACAG-3’) and 1 μM template-switch oligo (TSO) containing a 10-nt unique molecular identifier (UMI) (5’ -CTT TCC CTA CAC GAC GCT CTT CCG ATCT NNNNNNNNNN TAC rGrGrG/3phos/−3’). cDNA fragments of 150–300nt were size-selected on a 6% urea-PAGE gel, then amplified with Article Title: Cancer detection and classification using methylome analysis Article Snippet: Briefly, for MeDIP, the Diagenode MagMeDIP kit (Cat #C02010021) was used following the manufacturer's protocol with some modifications. .. The optimal number of cycles to amplify each library was determined through the use of qPCR, after which the samples were amplified using the KAPA HiFi Hotstart Mastermix and the Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA. Article Snippet: .. Preparation of Illumina libraries and NGS sequencing Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Article Title: HM-DyadCap – capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA Article Snippet: .. Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using Generated:Article Title: Epigenetic control of S100A8/A9-driven monocytic inflammation licenses anti-leukemic functionality of immature NK cells during hematopoietic stem cell differentiation Article Snippet: .. Libraries were generated from total RNA after poly(A)^+^ mRNA enrichment using the NEBNext Ultra II Directional RNA Library Prep Kit with Real-time Polymerase Chain Reaction:Article Title: Cancer detection and classification using methylome analysis Article Snippet: Briefly, for MeDIP, the Diagenode MagMeDIP kit (Cat #C02010021) was used following the manufacturer's protocol with some modifications. .. The optimal number of cycles to amplify each library was determined through the use of qPCR, after which the samples were amplified using the KAPA HiFi Hotstart Mastermix and the Concentration Assay:Article Title: Cancer detection and classification using methylome analysis Article Snippet: Briefly, for MeDIP, the Diagenode MagMeDIP kit (Cat #C02010021) was used following the manufacturer's protocol with some modifications. .. The optimal number of cycles to amplify each library was determined through the use of qPCR, after which the samples were amplified using the KAPA HiFi Hotstart Mastermix and the Hybridization:Article Title: deluxpore: a Nextflow pipeline for demultiplexing Illumina dual-indexed Nanopore libraries Article Snippet: .. As a solution, target enrichment can be performed on Illumina-indexed libraries, whereby dual indexes incorporated during library preparation allow samples to be pooled prior to capture hybridisation, after which the enriched pool is converted into a Nanopore-compatible library for MinION sequencing; a strategy recently applied to microbial gene capture using Next-Generation Sequencing:Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA. Article Snippet: .. Preparation of Illumina libraries and NGS sequencing Enriched DNA fragments were PCR amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, version 6.1_5/20) using |
